Linear scalability of virus production in the integrity® iCELLis® single-use fixed-bed bioreactors from bench to industrial scale

Introduction In order to maximize cell growth within a compact space and retain cells for easy medium exchange, the iCELLis bioreactors from ATMI LifeSciences contain macro-carriers trapped in a fixed-bed, creating a 3-D matrix within which cells adhere and replicate. These bioreactors also enable precise temperature, pH and dissolved oxygen control which cannot be done in 2-D cultures. The iCELLis technology can be used at small and large scales with straightforward process scale-up, easy single-use operations and minimal space requirement. Here we present a summary of adherent cell process development in iCELLis bioreactors, including: